abstract：:The rfb (O antigen) gene cluster of group C2 Salmonella differs from that of group B in a central region of 12.4 kb: we report the sequencing of this region of strain M67 (group C2) and a subsequent comparison with the central region of strain LT2 (group B). We find a block of seven open reading frames unique to group...

journal_title：Molecular microbiology

authors： Brown PK,Romana LK,Reeves PR

更新日期：1992-05-01 00:00:00

abstract：:Synthesis and secretion of the 110kDa haemolysin toxin of Escherichia coli and other pathogenic Gram-negative bacteria are governed by the four genes of the hly operon. We have identified, by transposon mutagenesis, an E. coli cellular locus, hlyT, required for the synthesis and secretion of haemolysin encoded in tran...

journal_title：Molecular microbiology

authors： Bailey MJ,Koronakis V,Schmoll T,Hughes C

更新日期：1992-04-01 00:00:00

abstract：:The structures of the choline-dependent pneumococcal murein hydrolases, LYTA amidase and CPL1 lysozyme, and the choline-independent CPL7 lysozyme were analysed by controlled proteolytic digestions. The trypsin cleavage of the CPL1 and CPL7 lysozymes produced two resistant polypeptides, F1 and F7 respectively, correspo...

journal_title：Molecular microbiology

authors： Sanz JM,Díaz E,García JL

更新日期：1992-04-01 00:00:00

abstract：:The kilB locus (which is unclonable in the absence of korB) of broad-host-range plasmid RK2 (60 kb) lies between the trfA operon (co-ordinates 16.4 to 18.2 kb), which encodes a protein essential for vegetative replication, and the Tra2 block of conjugative transfer genes (co-ordinates 20.0 to 27.0 kb). Promoter probe ...

journal_title：Molecular microbiology

authors： Motallebi-Veshareh M,Balzer D,Lanka E,Jagura-Burdzy G,Thomas CM

更新日期：1992-04-01 00:00:00

abstract：:The location of FtsQ, an Escherichia coli protein essential for cell division, is, under physiological conditions, in the cytoplasmic membrane facing towards the periplasmic space. An amino-proximal hydrophobic domain is required for FtsQ to reach its location and for its activity in the cell. Overexpression of modifi...

journal_title：Molecular microbiology

authors： Dopazo A,Palacios P,Sánchez M,Pla J,Vicente M

更新日期：1992-03-01 00:00:00

abstract：:The ctaD gene encoding subunit I of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% ide...

journal_title：Molecular microbiology

authors： Shapleigh JP,Gennis RB

更新日期：1992-03-01 00:00:00

abstract：:The Incl1 conjugative plasmid Collb-P9 carries a psiB gene that prevents induction of the SOS response in host bacteria. This locus is located 2.5 kb downstream of the ssb (single-stranded DNA-binding protein) gene in the leading region. This portion of Collb is strikingly similar to part of the leading region of the ...

journal_title：Molecular microbiology

authors： Jones AL,Barth PT,Wilkins BM

更新日期：1992-03-01 00:00:00

abstract：:The K1 killer toxin of Saccharomyces cerevisiae consists of 103- and 83-residue alpha and beta components whose derivation, from a 316-residue precursor preprotoxin, requires processing at the alpha N-terminus (after ProArg-44), the alpha C-terminus (after ArgArg-149) and at the beta N-terminus (after LysArg-233). The...

journal_title：Molecular microbiology

authors： Zhu YS,Zhang XY,Cartwright CP,Tipper DJ

更新日期：1992-02-01 00:00:00

abstract：:CS1 pili are filamentous proteinaceous appendages found on many enterotoxigenic Escherichia coli (ETEC) strains isolated from human diarrhoeal disease. They are thought to effect colonization of the upper intestine by facilitating binding to human ileal epithelial cells. We have identified a gene, cooB, which lies dir...

journal_title：Molecular microbiology

authors： Scott JR,Wakefield JC,Russell PW,Orndorff PE,Froehlich BJ

更新日期：1992-02-01 00:00:00

abstract：:Klebsiella pullulanase is a lipoprotein synthesized as a precursor with a signal peptide, which is processed by lipoprotein signal peptidase. To clarify the role of lipid modification of pullulanase, we purified lipid-modified wild-type and the unmodified (mutant) pullulanases and compared their properties. The Km and...

journal_title：Molecular microbiology

authors： Yamashita M,Nakagawa A,Katsuragi N,Murooka Y

更新日期：1992-02-01 00:00:00

abstract：:Previous studies with purified variants of the 434 repressor having different operator-binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 oper...

journal_title：Molecular microbiology

authors： Webster C,Merryweather A,Brammar W

更新日期：1992-02-01 00:00:00

abstract：:In response to recommendations from the Steering Committees responsible for co-ordination of World Health Organization programmes for research on the immunology of leprosy (IMMLEP) and tuberculosis (IMMTUB), a list was prepared summarizing the properties of mycobacterial proteins currently under investigation with res...

journal_title：Molecular microbiology

authors： Young DB,Kaufmann SH,Hermans PW,Thole JE

更新日期：1992-01-01 00:00:00

abstract：:Escherichia coli haemolysin (Hlya) is a pore-forming protein which belongs to the family of 'Repeat-toxins' (RTX) (Lo et al., 1987; Lally et al., 1989; Kraig et al., 1990). A model for the pore-forming structure of HlyA has been proposed (Ludwig et al., 1991) which consists of eight transmembrane segments all present ...

journal_title：Molecular microbiology

authors： Oropeza-Wekerle RL,Muller S,Briand JP,Benz R,Schmid A,Goebel W

更新日期：1992-01-01 00:00:00

abstract：:A range of circumstantial evidence suggests that in Streptomyces spp., genes required for vegetative growth do not contain the leucine codon TTA. Instead, the codon seems to be confined to a few genes necessary during differentiation, when the colonies begin to produce aerial hyphae and antibiotics. Thus, mutations in...

journal_title：Molecular microbiology

authors： Leskiw BK,Bibb MJ,Chater KF

更新日期：1991-12-01 00:00:00

abstract：:In this work we present the localization and characterization of the repl promoter (Prepl) and show aspects of the regulation. Comparison of Prepl with other autoregulated replication protein gene promoters revealed similarities, but Prepl differs from some of these characterized promoters in not being regulated by th...

journal_title：Molecular microbiology

authors： Gammie AE,Crosa JH

更新日期：1991-12-01 00:00:00

abstract：:In matings between Lactococcus lactis strains, the conjugative transposons Tn916 and Tn919 are found in the chromosome of the transconjugants in the same place as in the chromosome of the donor, indicating that no transposition has occurred. In agreement with this, the frequency of L. lactis transconjugants from intra...

journal_title：Molecular microbiology

authors： Bringel F,Van Alstine GL,Scott JR

更新日期：1991-12-01 00:00:00

abstract：:The NAC (nitrogen assimilation control) protein from Klebsiella aerogenes is a LysR-like regulator for transcription of several operons involved in nitrogen metabolism, and couples the transcription of these sigma 70-dependent operons to regulation by the sigma 54-dependent NTR system. NAC activates expression of oper...

journal_title：Molecular microbiology

authors： Bender RA

更新日期：1991-11-01 00:00:00

abstract：:DnaA protein (a trans-acting element) and its binding sequence, DnaA-box: (a cis-acting element) are two elements essential for the initiation of chromosomal replication in Escherichia coli and other enteric bacteria. Recently these two elements have been found to be conserved in three Gram-positive bacteria (Bacillus...

journal_title：Molecular microbiology

authors： Yoshikawa H,Ogasawara N

更新日期：1991-11-01 00:00:00

abstract：:Two distinct merR genes, which regulate expression of the mercuric ion resistance gene (mer), of Thiobacillus ferrooxidans strain E-15 have been cloned, sequenced and termed merR1 and merR2. As a result of gene walking around two merR genes, it was found that these two genes were quite close in distance. The nucleotid...

journal_title：Molecular microbiology

authors： Inoue C,Sugawara K,Kusano T

更新日期：1991-11-01 00:00:00

abstract：:By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open readin...

journal_title：Molecular microbiology

authors： Torres L,Martín H,García-Saez MI,Arroyo J,Molina M,Sánchez M,Nombela C

更新日期：1991-11-01 00:00:00

abstract：:Crown gall tumorigenesis by Agrobacterium tumefaciens requires the co-ordinate transcriptional induction of a set of pathogenesis genes. At least three classes of environmental stimuli act synergistically to induce these genes: (i) monocyclic aromatic hydrocarbons such as acetosyringone, coniferyl alcohol, and vanilli...

journal_title：Molecular microbiology

authors： Winans SC

更新日期：1991-10-01 00:00:00

abstract：:The bvg locus contains two genes, bvgA and bvgS, which control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli. We determined the nucleotide sequence of the bvg loci of Bor...

journal_title：Molecular microbiology

authors： Aricò B,Scarlato V,Monack DM,Falkow S,Rappuoli R

更新日期：1991-10-01 00:00:00

abstract：:The cytochrome d complex of Escherichia coli is a heterodimer located in the bacterial cytoplasmic membrane, where it functions as a terminal oxidase of the aerobic respiratory chain. The topology of each of the two subunits of the cytochrome d complex was analysed by the genetic method involving alkaline phosphatase ...

journal_title：Molecular microbiology

authors： Newton G,Yun CH,Gennis RB

更新日期：1991-10-01 00:00:00

abstract：:The regulation of gene expression by the two-component regulatory system PhoP/PhoQ is necessary for Salmonella typhimurium survival within macrophages, defensin resistance, acid resistance, and murine typhoid fever pathogenesis. Salmonella experience multiple environments during mammalian infection and survival requir...

journal_title：Molecular microbiology

authors： Miller SI

更新日期：1991-09-01 00:00:00

abstract：:A 2 kb DNA fragment isolated from a cosmid library of Aquaspirillum magnetotacticum strain MS-1 complements the aromatic-metabolite requirements and iron-uptake deficiencies of Escherichia coli and Salmonella typhimurium strains that lack a functional aroD (biosynthetic dehydrodquinase) sequence. All recombinant cosmi...

journal_title：Molecular microbiology

authors： Berson AE,Hudson DV,Waleh NS

更新日期：1991-09-01 00:00:00

abstract：:Escherichia coli K-12 strain PS1-28-37 carries the multicopy plasmid pPSO28-37 containing a DNA fragment coding for two of the proteins that enable bacteria to utilize sucrose as sole carbon source. One of the different gene products of the plasmid is the outer membrane protein, ScrY. This protein was isolated and pur...

journal_title：Molecular microbiology

authors： Schülein K,Schmid K,Benzl R

更新日期：1991-09-01 00:00:00

abstract：:The porins of Gram-negative bacteria are responsible for the 'molecular sieve' properties of the outer membrane. They form large water-filled channels which allow the diffusion of hydrophilic molecules into the periplasmic space. Owing to the strong hydrophilicity of their amino acid sequence and the nature of their s...

journal_title：Molecular microbiology

authors： Jeanteur D,Lakey JH,Pattus F

更新日期：1991-09-01 00:00:00

abstract：:Two previously identified Bacillus subtilis DNA segments, dciA and dciB, whose transcripts accumulate very rapidly after induction of sporulation, were found in the same 6.2 kb transcription unit, now known as the dciA operon. Analysis of the sequence of the dciA operon showed that its putative products are homologous...

journal_title：Molecular microbiology

authors： Mathiopoulos C,Mueller JP,Slack FJ,Murphy CG,Patankar S,Bukusoglu G,Sonenshein AL

更新日期：1991-08-01 00:00:00

abstract：:The chromosomal gef gene of Escherichia coli is a member of the gef gene family which encodes strongly toxic proteins of about 50 amino acids. We demonstrate here that the Gef protein is detectable by anti-peptide antibodies. Furthermore, we show that Gef is anchored in the cytoplasmic membrane by the N-terminal part ...

journal_title：Molecular microbiology

authors： Poulsen LK,Refn A,Molin S,Andersson P

更新日期：1991-07-01 00:00:00

abstract：:Differential expression of the genes expressing Pap pili in Escherichia coli was suggested to involve mRNAs with different stabilities. As the result of a post-transcriptional processing event, a papA gene-specific mRNA product (mRNA-A) accumulates in large excess relative to the primary mRNA-BA transcript. Our result...

journal_title：Molecular microbiology

authors： Nilsson P,Uhlin BE

更新日期：1991-07-01 00:00:00

abstract：:Entamoeba histolytica cells secrete electron-dense granules (EDGs) that have collagenase activity. To study the possible involvement of calmodulin (CaM) on EDG secretion, the effect of several CaM antagonists (TFP, R24571, W-7, W-5, dibucaine and DL-propranolol) was tested on this cellular function. Except for W-5 and...

journal_title：Molecular microbiology

authors： Muñoz Mde L,Moreno MA,Pérez-Garcia JN,Tovar GR,Hernandez VI

更新日期：1991-07-01 00:00:00

abstract：:We have cloned a gene encoding a polygalacturonase (PG) in the filamentous fungus Aspergillus niger RH5344. The structural gene comprises 1141 bp coding for 362 amino acids and the open reading frame is disrupted by one intron of 52 bp. Eukaryotic consensus sequences for transcription regulation are found only in devi...

journal_title：Molecular microbiology

authors： Ruttkowski E,Khanh NQ,Wientjes FJ,Gottschalk M

更新日期：1991-06-01 00:00:00

abstract：:A 2.6 kb ClaI-BamHI DNA fragment of megaplasmid 2 of Rhizobium meliloti 2011 was found to carry genes involved in exopolysaccharide synthesis and infection of alfalfa nodules. The analysis of the nucleotide sequence of this DNA fragment revealed the existence of two open reading frames (ORFs) running in opposite direc...

journal_title：Molecular microbiology

authors： Buendia AM,Enenkel B,Köplin R,Niehaus K,Arnold W,Pühler A

更新日期：1991-06-01 00:00:00

abstract：:The overall transcription of DNA has previously been demonstrated to proceed at extremely high levels of accuracy. We review the evidence that the process of transcription is subject to proof-reading in the Hopfield sense. In addition, we speculate that the proof-reading activity associated with transcription is subje...

journal_title：Molecular microbiology

authors： Libby RT,Gallant JA

更新日期：1991-05-01 00:00:00

abstract：:A 34 kb fragment of the Nocardia lactamdurans DNA carrying the cluster of early cephamycin biosynthetic genes was cloned in lambda EMBL3 by hybridization with probes internal to the pcbAB and pcbC genes of Penicillium chrysogenum and Streptomyces griseus. The pcbAB and pcbC genes were found to be closely linked togeth...

journal_title：Molecular microbiology

authors： Coque JJ,Martín JF,Calzada JG,Liras P

更新日期：1991-05-01 00:00:00

abstract：:External factors regulate the formation of pigment protein complexes in facultatively photosynthetic bacteria. The puf operon of Rhodobacter capsulatus encodes the pigment binding proteins of the reaction centre and light-harvesting I complex. Here we demonstrate that a single base-pair exchange within a sequence of d...

journal_title：Molecular microbiology

authors： Klug G,Gad'on N,Jock S,Narro ML

更新日期：1991-05-01 00:00:00

abstract：:We report here the formation of symbiotic plasmids (pSyms), by genetic recombination between rearranged pSyms, which lack symbiotic information, and resistance plasmids carrying parts of different symbiotic plasmids (R's). This recombination was found to occur both between plasmids derived from different Rhizobium pha...

journal_title：Molecular microbiology

authors： Soberón-Chávez G,Nájera R,Espín G,Moreno S

更新日期：1991-04-01 00:00:00

abstract：:Copy-number mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B (MLS) resistance plasmid pT48 were isolated by their resistance to the non-inducing macrolide, tylosin. One mutant plasmid, pcopD3, showed a three- to five-fold cis-dominant increase in copy number, and nucleotide sequence analysis reve...

journal_title：Molecular microbiology

authors： Catchpole I,Dyke KG

更新日期：1991-04-01 00:00:00

abstract：:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

journal_title：Molecular microbiology

authors： Kaminski PA,Elmerich C

更新日期：1991-03-01 00:00:00

abstract：:The Escherichia coli treA gene encodes an osmotically inducible periplasmic trehalase. A strain carrying a treA-lacZ transcriptional fusion was constructed. The beta-galactosidase activity produced in this strain growing exponentially in a medium of high osmotic pressure was 10-fold higher than that produced in a medi...

journal_title：Molecular microbiology

authors： Repoila F,Gutierrez C

更新日期：1991-03-01 00:00:00